Human ADIPOR2 (Adiponectin Receptor 2) CLIA Kit (E-CL-H0227)

Name: Human ADIPOR2 (Adiponectin Receptor 2) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0227
Price: 180.0 USD
Availability: InStock

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Human ADIPOR2 (Adiponectin Receptor 2) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	0.09 ng/mL
Detection Range	0.16-10 ng/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human ADIPOR2 in samples.No significant cross-reactivity or interference between Human ADIPOR2 and analogues was observed
Recovery	80%-120%
Sample Type	Tissue homogenates, cell lysates and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
10	33226.000	33340.000	33283.000	33249
5	14940.000	17664.000	16302.000	16268
2.5	7872.000	7728.000	7800.000	7766
1.25	3532.000	4138.000	3835.000	3801
0.63	1834.000	1732.000	1783.000	1749
0.31	925.000	815.000	870.000	836
0.16	360.000	366.000	363.000	329
0	33.000	35.000	34.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	0.550	1.150	4.890	0.540	1.220	4.860
Standard deviation	0.040	0.100	0.520	0.050	0.100	0.400
CV（%）	7.270	8.700	10.630	9.260	8.200	8.230

Recovery

The recovery of Human ADIPOR2 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	95-111	101
EDTA plasma （n=8）	96-112	104
Cell culture media （n=8）	91-102	97

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human ADIPOR2 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	102-116	93-109	99-113
1:2	Average	108	101	105
1:4	Range	99-118	99-111	95-109
1:4	Average	107	104	101
1:8	Range	95-110	83-97	87-99
1:8	Average	101	90	94

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ADIPOR2. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ADIPOR2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ADIPOR2, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human ADIPOR2. You can calculate the concentration of Human ADIPOR2 in the samples by comparing the RLU value of the samples to the standard curve.

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