Human AGR2 (Anterior Gradient Protein 2) CLIA Kit (E-CL-H0243)

Name: Human AGR2 (Anterior Gradient Protein 2) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0243
Price: 180.0 USD
Availability: InStock

Copy the product info.

Citations ({{ citationsPage.numTotal }}) Manual MSDS

Human AGR2 (Anterior Gradient Protein 2) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

Bulk request

Add to cart

For research use only. Order now, ship in 3 days

Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human AGR2 in samples.No significant cross-reactivity or interference between Human AGR2 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	55819.000	61473.000	58646.000	58611
1000	22348.000	27152.000	24750.000	24715
500	12147.000	11141.000	11644.000	11609
250	5565.000	5683.000	5624.000	5589
125	2905.000	2799.000	2852.000	2817
62.5	1495.000	1315.000	1405.000	1370
31.25	655.000	729.000	692.000	657
0	35.000	35.000	35.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	110.650	170.570	861.730	114.700	186.330	864.080
Standard deviation	9.960	19.310	63.340	12.150	19.880	57.550
CV（%）	9.000	11.320	7.350	10.590	10.670	6.660

Recovery

The recovery of Human AGR2 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	89-101	94
EDTA plasma （n=8）	84-98	91
Cell culture media （n=8）	92-109	99

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human AGR2 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	86-101	100-114	100-116
1:2	Average	93	106	108
1:4	Range	89-102	87-103	95-109
1:4	Average	95	94	100
1:8	Range	88-103	99-114	102-115
1:8	Average	96	105	109

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human AGR2. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AGR2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AGR2, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human AGR2. You can calculate the concentration of Human AGR2 in the samples by comparing the RLU value of the samples to the standard curve.

Background