Human AGT (Angiotensinogen) ELISA Kit (E-EL-H6310)

Name: Human AGT (Angiotensinogen) ELISA Kit
Brand: Elabscience
SKU: E-EL-H6310
Price: 150.0 USD
Availability: InStock

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Human AGT (Angiotensinogen) ELISA Kit - 1

Size	Price	Qty
96T	$ 609.00	- +
48T	$ 487.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	9.38 ng/mL
Detection Range	15.63-1000 ng/mL
Sample Volume	100 μL
Manual Operation Time	1 h
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes AGT in samples.No significant cross-reactivity or interference between AGT and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
1000	2.050	2.084	2.067	1.993
500	1.373	1.429	1.401	1.327
250	0.881	0.839	0.860	0.786
125	0.476	0.468	0.472	0.398
62.5	0.255	0.265	0.260	0.186
31.25	0.171	0.169	0.170	0.096
15.63	0.116	0.124	0.120	0.046
0	0.071	0.077	0.074	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	48.060	130.200	421.870	49.290	134.480	431.440
Standard deviation	2.870	6.550	27.930	4.050	8.100	32.190
CV（%）	5.980	5.030	6.620	8.220	6.020	7.460

Recovery

The recovery of Human AGT was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Cell culture media （n=8）	95-110	101
Serum （n=8）	91-105	96
EDTA plasma （n=8）	89-103	96

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human AGT and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	85-95	85-101	95-109
1:2	Average	90	92	102
1:4	Range	84-96	82-94	90-105
1:4	Average	90	89	98
1:8	Range	83-94	87-99	94-110
1:8	Average	89	93	101

Stability

Each kit batch is subjected to accelerated stability testing and real-time stability monitoring. Sample performance is evaluated after storage at 37 °C for 10 days to assess the impact of elevated temperature on assay reliability and reagent integrity.

/	Variation range of 37°C mean concentration / 2-8°C mean
Sample 1（n=16）	99.10-114.29
Sample 2（n=16）	85.54-106.02

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to AGT . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for AGT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain AGT , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of AGT . You can calculate the concentration of AGT in the samples by comparing the OD of the samples to the standard curve.

Background

The protein encoded by the AGT gene is the angiotensinogen precursor, which is expressed in the liver and cleaved by reninase in response to a decrease in blood pressure. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure and in the pathogenesis of essential hypertension and preeclampsia. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease.