Human ALCAM (Activated Leukocyte Cell Adhesion Molecule) CLIA Kit (E-CL-H0247)

Name: Human ALCAM (Activated Leukocyte Cell Adhesion Molecule) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0247
Price: 180.0 USD
Availability: InStock

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Human ALCAM (Activated Leukocyte Cell Adhesion Molecule) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	9.38 pg/mL
Detection Range	15.63-1000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human ALCAM in samples.No significant cross-reactivity or interference between Human ALCAM and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
1000	57545.000	57673.000	57609.000	57578
500	22864.000	26580.000	24722.000	24691
250	11424.000	10252.000	10838.000	10807
125	5006.000	5392.000	5199.000	5168
62.5	2683.000	2603.000	2643.000	2612
31.25	1454.000	1330.000	1392.000	1361
15.63	734.000	780.000	757.000	726
0	30.000	32.000	31.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	48.180	117.820	396.200	51.340	120.920	410.080
Standard deviation	5.820	13.020	40.610	6.480	13.710	33.910
CV（%）	12.080	11.050	10.250	12.620	11.340	8.270

Recovery

The recovery of Human ALCAM was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	89-101	94
EDTA plasma （n=8）	90-101	95
Cell culture media （n=8）	95-111	102

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human ALCAM and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	88-100	86-100	94-106
1:2	Average	94	92	100
1:4	Range	98-110	90-103	87-102
1:4	Average	104	95	94
1:8	Range	91-106	93-106	99-113
1:8	Average	98	99	105

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ALCAM. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ALCAM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ALCAM, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human ALCAM. You can calculate the concentration of Human ALCAM in the samples by comparing the RLU value of the samples to the standard curve.

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