Human ANGPT4 (Angiopoietin 4) CLIA Kit (E-CL-H0266)

Name: Human ANGPT4 (Angiopoietin 4) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0266
Price: 180.0 USD
Availability: InStock

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Human ANGPT4 (Angiopoietin 4) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human ANGPT4 in samples.No significant cross-reactivity or interference between Human ANGPT4 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	55241.000	64745.000	59993.000	59969
1000	24941.000	25825.000	25383.000	25359
500	12504.000	11690.000	12097.000	12073
250	5155.000	5497.000	5326.000	5302
125	2946.000	2592.000	2769.000	2745
62.5	1508.000	1356.000	1432.000	1408
31.25	677.000	769.000	723.000	699
0	23.000	25.000	24.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	102.860	300.310	973.570	99.810	291.760	1051.180
Standard deviation	11.510	28.500	62.600	11.260	23.310	87.880
CV（%）	11.190	9.490	6.430	11.280	7.990	8.360

Recovery

The recovery of Human ANGPT4 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	98-112	104
EDTA plasma （n=8）	89-101	94
Cell culture media （n=8）	100-116	106

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human ANGPT4 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	96-113	88-102	86-99
1:2	Average	103	93	92
1:4	Range	85-100	92-108	95-108
1:4	Average	92	99	100
1:8	Range	95-107	96-113	100-117
1:8	Average	101	104	108

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANGPT4. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANGPT4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ANGPT4, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human ANGPT4. You can calculate the concentration of Human ANGPT4 in the samples by comparing the RLU value of the samples to the standard curve.

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