Human Bax (Bcl-2 Associated X Protein) CLIA Kit (E-CL-H0440)

Name: Human Bax (Bcl-2 Associated X Protein) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0440
Price: 180.0 USD
Availability: InStock

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Human Bax (Bcl-2 Associated X Protein) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	0.09 ng/mL
Detection Range	0.16-10 ng/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human BAX in samples.No significant cross-reactivity or interference between Human BAX and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
10	31167.000	35283.000	33225.000	33198
5	15731.000	17869.000	16800.000	16773
2.5	8640.000	7362.000	8001.000	7974
1.25	3825.000	4177.000	4001.000	3974
0.63	1901.000	1699.000	1800.000	1773
0.31	958.000	850.000	904.000	877
0.16	346.000	394.000	370.000	343
0	26.000	28.000	27.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	0.540	1.130	4.190	0.550	1.020	4.400
Standard deviation	0.050	0.080	0.360	0.070	0.090	0.370
CV（%）	9.260	7.080	8.590	12.730	8.820	8.410

Recovery

The recovery of Human BAX was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	99-113	107
EDTA plasma （n=8）	102-119	108
Cell culture media （n=8）	97-107	102

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human BAX and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	102-116	87-99	95-111
1:2	Average	109	94	103
1:4	Range	88-103	99-110	95-111
1:4	Average	94	105	102
1:8	Range	99-116	84-96	97-115
1:8	Average	107	91	105

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human BAX. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BAX and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BAX, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human BAX. You can calculate the concentration of Human BAX in the samples by comparing the RLU value of the samples to the standard curve.

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