Human bFGF/FGF2 (Basic Fibroblast Growth Factor) CLIA Kit (E-CL-H0394)

Name: Human bFGF/FGF2 (Basic Fibroblast Growth Factor) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0394
Price: 180.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Human bFGF/FGF2 (Basic Fibroblast Growth Factor) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	7.5 pg/mL
Detection Range	12.5-800 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human bFGF/FGF2 in samples.No significant cross-reactivity or interference between Human bFGF/FGF2 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
800	52504.000	59152.000	55828.000	55796
400	21449.000	23233.000	22341.000	22309
200	11186.000	9690.000	10438.000	10406
100	4930.000	5046.000	4988.000	4956
50	2310.000	2260.000	2285.000	2253
25	1209.000	1047.000	1128.000	1096
12.5	507.000	573.000	540.000	508
0	31.000	33.000	32.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	39.160	84.030	280.730	39.060	77.900	290.550
Standard deviation	4.970	5.980	32.060	4.660	8.370	20.340
CV（%）	12.690	7.120	11.420	11.930	10.740	7.000

Recovery

The recovery of Human bFGF was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	91-103	97
EDTA plasma （n=8）	98-115	105
Cell culture media （n=8）	99-117	107

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human bFGF and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	90-106	85-100	93-107
1:2	Average	97	91	99
1:4	Range	93-106	94-109	97-112
1:4	Average	98	101	105
1:8	Range	93-107	99-113	94-108
1:8	Average	99	105	100

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human bFGF/FGF2. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human bFGF/FGF2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human bFGF/FGF2, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human bFGF/FGF2. You can calculate the concentration of Human bFGF/FGF2 in the samples by comparing the RLU value of the samples to the standard curve.

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