Human BPI (Bactericidal/Permeability Increasing Protein) CLIA Kit (E-CL-H0435)
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For research use only.
Product Summary
| Sensitivity | 7.5 pg/mL |
| Detection Range | 12.5-800 pg/mL |
| Sample Volume | 100 μL |
| Total Assay Time | 3 h 30 min |
| Reactivity | Human |
| Specificity | This kit recognizes Human BPI in samples.No significant cross-reactivity or interference between Human BPI and analogues was observed |
| Recovery | 80%-120% |
| Sample Type | Serum, plasma and other biological fluids |
| Detection Method | Chemiluminescence |
| Assay Type | Sandwich-CLIA |
| Size | 96T / 48T / 24T / 96T*5 / 96T*10 |
| Storage | 2-8℃/-20℃ |
| Expiration Date | 6 months |
Performance
| Recovery |
The recovery of Human BPI was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.
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| Linearity |
Linearity of the assay was evaluated by spiking samples with high concentrations of Human BPI and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range.
The measured values were then compared to the expected concentrations to assess the linearity of response.
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Test Principle
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human BPI. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BPI and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BPI, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human BPI. You can calculate the concentration of Human BPI in the samples by comparing the RLU value of the samples to the standard curve.
Background
| Gene ID | 671 |
| Uniport ID | P17213 |
| Research Area | Cardiovascular , Immunology , Microbiology |
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