Human BPI (Bactericidal/Permeability Increasing Protein) CLIA Kit (E-CL-H0435)

Name: Human BPI (Bactericidal/Permeability Increasing Protein) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0435
Price: 180.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Human BPI (Bactericidal/Permeability Increasing Protein) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	7.5 pg/mL
Detection Range	12.5-800 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human BPI in samples.No significant cross-reactivity or interference between Human BPI and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
800	54600.000	60902.000	57751.000	57713
400	23442.000	23448.000	23445.000	23407
200	11001.000	10301.000	10651.000	10613
100	4328.000	4904.000	4616.000	4578
50	2387.000	2211.000	2299.000	2261
25	1158.000	1094.000	1126.000	1088
12.5	520.000	580.000	550.000	512
0	38.000	38.000	38.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	38.220	95.590	360.560	34.450	90.490	343.690
Standard deviation	4.350	6.740	22.210	4.470	10.410	38.600
CV（%）	11.380	7.050	6.160	12.980	11.500	11.230

Recovery

The recovery of Human BPI was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	92-106	98
EDTA plasma （n=8）	91-102	97
Cell culture media （n=8）	93-106	98

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human BPI and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	88-100	92-105	92-105
1:2	Average	95	98	98
1:4	Range	93-105	91-107	90-103
1:4	Average	99	98	96
1:8	Range	103-117	88-102	85-97
1:8	Average	109	94	90

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human BPI. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BPI and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BPI, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human BPI. You can calculate the concentration of Human BPI in the samples by comparing the RLU value of the samples to the standard curve.

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