Human CG (Chorionic Gonadotropin) CLIA Kit (E-CL-H0160)

Name: Human CG (Chorionic Gonadotropin) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0160
Price: 180.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Human CG (Chorionic Gonadotropin) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	0.38 ng/mL
Detection Range	0.63-40 ng/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human CG in samples.No significant cross-reactivity or interference between Human CG and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
40	37492.000	42692.000	40092.000	40053
20	13368.000	15576.000	14472.000	14433
10	6792.000	6466.000	6629.000	6590
5	2927.000	3301.000	3114.000	3075
2.5	1809.000	1555.000	1682.000	1643
1.25	1000.000	988.000	994.000	955
0.63	599.000	669.000	634.000	595
0	38.000	40.000	39.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	1.870	3.530	18.270	1.980	3.820	18.350
Standard deviation	0.210	0.400	1.570	0.220	0.430	1.550
CV（%）	11.230	11.330	8.590	11.110	11.260	8.450

Recovery

The recovery of Human CG was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	85-98	91
EDTA plasma （n=8）	96-111	102
Cell culture media （n=8）	97-112	105

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human CG and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	92-106	102-115	98-116
1:2	Average	98	108	107
1:4	Range	94-111	95-110	92-104
1:4	Average	101	103	97
1:8	Range	86-99	102-116	91-105
1:8	Average	91	108	98

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CG. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CG, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human CG. You can calculate the concentration of Human CG in the samples by comparing the RLU value of the samples to the standard curve.

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