Human CXCR3 (Chemokine CXC Receptor 3) CLIA Kit (E-CL-H0592)

Name: Human CXCR3 (Chemokine CXC Receptor 3) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0592
Price: 180.0 USD
Availability: InStock

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Human CXCR3 (Chemokine CXC Receptor 3) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human CXCR3 in samples.No significant cross-reactivity or interference between Human CXCR3 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	51390.000	62764.000	57077.000	57046
1000	22375.000	26517.000	24446.000	24415
500	11705.000	11545.000	11625.000	11594
250	4946.000	5674.000	5310.000	5279
125	2833.000	2809.000	2821.000	2790
62.5	1404.000	1334.000	1369.000	1338
31.25	673.000	701.000	687.000	656
0	30.000	32.000	31.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	110.690	194.550	842.020	110.240	180.000	856.080
Standard deviation	11.180	18.400	56.670	9.890	15.140	88.090
CV（%）	10.100	9.460	6.730	8.970	8.410	10.290

Recovery

The recovery of Human CXCR3 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	95-107	100
EDTA plasma （n=8）	86-99	93
Cell culture media （n=8）	100-114	106

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human CXCR3 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	93-108	96-113	86-99
1:2	Average	101	104	91
1:4	Range	102-115	93-107	101-115
1:4	Average	109	99	109
1:8	Range	92-108	95-109	96-113
1:8	Average	100	102	103

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CXCR3. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CXCR3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CXCR3, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human CXCR3. You can calculate the concentration of Human CXCR3 in the samples by comparing the RLU value of the samples to the standard curve.

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