Human DβH (Dopamine-β-Hydroxylase) CLIA Kit (E-CL-H0797)

Name: Human DβH (Dopamine-β-Hydroxylase) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0797
Price: 180.0 USD
Availability: InStock

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Human DβH (Dopamine-β-Hydroxylase) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	0.19 ng/mL
Detection Range	0.31-20 ng/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human DβH in samples.No significant cross-reactivity or interference between Human DβH and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
20	33607.000	35641.000	34624.000	34599
10	12865.000	13733.000	13299.000	13274
5	5703.000	5315.000	5509.000	5484
2.5	2622.000	2802.000	2712.000	2687
1.25	1582.000	1432.000	1507.000	1482
0.63	933.000	825.000	879.000	854
0.31	626.000	698.000	662.000	637
0	24.000	26.000	25.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	0.920	2.220	6.940	0.860	2.430	7.090
Standard deviation	0.120	0.180	0.800	0.110	0.230	0.480
CV（%）	13.040	8.110	11.530	12.790	9.470	6.770

Recovery

The recovery of Human DβH was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	93-107	101
EDTA plasma （n=8）	99-111	105
Cell culture media （n=8）	90-106	97

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human DβH and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	102-117	95-111	87-99
1:2	Average	109	101	92
1:4	Range	94-106	99-113	98-117
1:4	Average	100	107	106
1:8	Range	88-100	87-100	88-101
1:8	Average	94	93	93

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human DβH. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DβH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DβH, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human DβH. You can calculate the concentration of Human DβH in the samples by comparing the RLU value of the samples to the standard curve.

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