Human DAO (Diamine Oxidase) CLIA Kit (E-CL-H0805)

Name: Human DAO (Diamine Oxidase) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0805
Price: 180.0 USD
Availability: InStock

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Human DAO (Diamine Oxidase) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	0.47 ng/mL
Detection Range	0.78-50 ng/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human DAO in samples.No significant cross-reactivity or interference between Human DAO and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
50	37595.000	38587.000	38091.000	38063
25	13953.000	16237.000	15095.000	15067
12.5	7590.000	7244.000	7417.000	7389
6.25	3346.000	3720.000	3533.000	3505
3.13	2025.000	1755.000	1890.000	1862
1.56	1089.000	1015.000	1052.000	1024
0.78	711.000	741.000	726.000	698
0	27.000	29.000	28.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	2.490	4.700	17.200	2.320	4.230	18.260
Standard deviation	0.230	0.520	1.090	0.260	0.310	1.160
CV（%）	9.240	11.060	6.340	11.210	7.330	6.350

Recovery

The recovery of Human DAO was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	95-111	101
EDTA plasma （n=8）	89-101	96
Cell culture media （n=8）	96-114	104

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human DAO and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	102-116	95-106	100-117
1:2	Average	108	101	108
1:4	Range	85-98	91-107	90-101
1:4	Average	90	98	95
1:8	Range	99-113	103-116	95-109
1:8	Average	105	109	103

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human DAO. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DAO and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DAO, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human DAO. You can calculate the concentration of Human DAO in the samples by comparing the RLU value of the samples to the standard curve.

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