Human DAT (Dopamine Transporter) CLIA Kit (E-CL-H0799)

Name: Human DAT (Dopamine Transporter) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0799
Price: 180.0 USD
Availability: InStock

Copy the product info.

Citations ({{ citationsPage.numTotal }}) Manual MSDS

Human DAT (Dopamine Transporter) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

Bulk request

Add to cart

For research use only. Order now, ship in 3 days

Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human DAT in samples.No significant cross-reactivity or interference between Human DAT and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	56448.000	62208.000	59328.000	59307
1000	22619.000	26579.000	24599.000	24578
500	11444.000	10788.000	11116.000	11095
250	5301.000	5517.000	5409.000	5388
125	2830.000	2536.000	2683.000	2662
62.5	1389.000	1379.000	1384.000	1363
31.25	660.000	724.000	692.000	671
0	20.000	22.000	21.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	105.610	311.520	799.850	104.500	281.060	863.900
Standard deviation	12.170	30.090	90.140	8.520	23.270	71.100
CV（%）	11.520	9.660	11.270	8.150	8.280	8.230

Recovery

The recovery of Human DAT was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	92-105	98
EDTA plasma （n=8）	87-98	92
Cell culture media （n=8）	86-98	93

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human DAT and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	100-113	103-116	85-100
1:2	Average	107	110	91
1:4	Range	94-110	87-100	103-115
1:4	Average	101	92	109
1:8	Range	92-107	89-103	93-106
1:8	Average	100	96	98

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human DAT. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DAT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DAT, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human DAT. You can calculate the concentration of Human DAT in the samples by comparing the RLU value of the samples to the standard curve.

Background