Human DDC (Dopamine Decarboxylase) CLIA Kit (E-CL-H0798)

Name: Human DDC (Dopamine Decarboxylase) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0798
Price: 180.0 USD
Availability: InStock

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Human DDC (Dopamine Decarboxylase) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human DDC in samples.No significant cross-reactivity or interference between Human DDC and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	55592.000	57778.000	56685.000	56655
1000	24823.000	26947.000	25885.000	25855
500	12373.000	11289.000	11831.000	11801
250	5456.000	5736.000	5596.000	5566
125	2934.000	2566.000	2750.000	2720
62.5	1451.000	1403.000	1427.000	1397
31.25	677.000	689.000	683.000	653
0	29.000	31.000	30.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	100.820	236.580	706.220	97.540	220.620	756.440
Standard deviation	12.890	17.460	54.100	8.550	20.940	47.660
CV（%）	12.790	7.380	7.660	8.770	9.490	6.300

Recovery

The recovery of Human DDC was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	92-109	99
EDTA plasma （n=8）	101-116	108
Cell culture media （n=8）	99-114	104

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human DDC and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	84-98	99-111	94-109
1:2	Average	90	105	99
1:4	Range	88-101	99-117	89-104
1:4	Average	94	107	96
1:8	Range	89-102	89-105	83-95
1:8	Average	94	96	90

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human DDC. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DDC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DDC, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human DDC. You can calculate the concentration of Human DDC in the samples by comparing the RLU value of the samples to the standard curve.

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