Human EPOR (Erythropoietin Receptor) CLIA Kit (E-CL-H0905)

Name: Human EPOR (Erythropoietin Receptor) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0905
Price: 180.0 USD
Availability: InStock

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Human EPOR (Erythropoietin Receptor) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human EPOR in samples.No significant cross-reactivity or interference between Human EPOR and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	57046.000	59050.000	58048.000	58022
1000	23373.000	27011.000	25192.000	25166
500	12387.000	11757.000	12072.000	12046
250	5085.000	5763.000	5424.000	5398
125	2851.000	2777.000	2814.000	2788
62.5	1522.000	1346.000	1434.000	1408
31.25	681.000	781.000	731.000	705
0	26.000	26.000	26.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	105.000	196.980	806.380	107.930	180.720	763.060
Standard deviation	11.320	22.670	64.110	12.760	18.400	77.760
CV（%）	10.780	11.510	7.950	11.820	10.180	10.190

Recovery

The recovery of Human EPOR was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	96-111	104
EDTA plasma （n=8）	84-97	91
Cell culture media （n=8）	101-117	107

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human EPOR and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	95-106	87-102	87-104
1:2	Average	101	94	95
1:4	Range	91-107	88-101	96-109
1:4	Average	97	96	102
1:8	Range	103-119	89-103	91-103
1:8	Average	109	96	96

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human EPOR. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human EPOR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human EPOR, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human EPOR. You can calculate the concentration of Human EPOR in the samples by comparing the RLU value of the samples to the standard curve.

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