Human ESM1 (Endothelial Cell Specific Molecule 1) CLIA Kit (E-CL-H1000)

Name: Human ESM1 (Endothelial Cell Specific Molecule 1) CLIA Kit
Brand: Elabscience
SKU: E-CL-H1000
Price: 180.0 USD
Availability: InStock

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Human ESM1 (Endothelial Cell Specific Molecule 1) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	4.69 pg/mL
Detection Range	7.81-500 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human ESM1 in samples.No significant cross-reactivity or interference between Human ESM1 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
500	52477.000	59597.000	56037.000	56002
250	21877.000	23659.000	22768.000	22733
125	10671.000	9129.000	9900.000	9865
62.5	4296.000	5034.000	4665.000	4630
31.25	2651.000	2277.000	2464.000	2429
15.63	1353.000	1155.000	1254.000	1219
7.81	729.000	801.000	765.000	730
0	33.000	37.000	35.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	23.540	45.150	245.070	22.750	43.280	231.410
Standard deviation	3.020	3.900	19.880	2.810	3.140	25.410
CV（%）	12.830	8.640	8.110	12.350	7.260	10.980

Recovery

The recovery of Human ESM1 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	99-115	105
EDTA plasma （n=8）	90-104	95
Cell culture media （n=8）	98-113	104

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human ESM1 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	100-115	87-98	95-112
1:2	Average	108	92	102
1:4	Range	95-110	93-109	84-97
1:4	Average	101	100	91
1:8	Range	92-107	98-110	100-117
1:8	Average	98	103	107

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ESM1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ESM1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ESM1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human ESM1. You can calculate the concentration of Human ESM1 in the samples by comparing the RLU value of the samples to the standard curve.

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