Human GAD1 (Glutamate Decarboxylase 1) CLIA Kit (E-CL-H1400)

Name: Human GAD1 (Glutamate Decarboxylase 1) CLIA Kit
Brand: Elabscience
SKU: E-CL-H1400
Price: 180.0 USD
Availability: InStock

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Human GAD1 (Glutamate Decarboxylase 1) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	0.19 ng/mL
Detection Range	0.31-20 ng/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human GAD1 in samples.No significant cross-reactivity or interference between Human GAD1 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
20	34670.000	39038.000	36854.000	36833
10	12866.000	13682.000	13274.000	13253
5	5803.000	5445.000	5624.000	5603
2.5	2373.000	2793.000	2583.000	2562
1.25	1507.000	1319.000	1413.000	1392
0.63	939.000	821.000	880.000	859
0.31	646.000	656.000	651.000	630
0	20.000	22.000	21.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	0.990	2.400	9.360	1.010	2.230	10.040
Standard deviation	0.120	0.280	0.900	0.100	0.160	1.130
CV（%）	12.120	11.670	9.620	9.900	7.170	11.250

Recovery

The recovery of Human GAD1 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	95-113	103
EDTA plasma （n=8）	98-114	105
Cell culture media （n=8）	102-114	108

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human GAD1 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	101-121	85-101	98-111
1:2	Average	110	92	104
1:4	Range	99-111	98-110	97-113
1:4	Average	105	104	105
1:8	Range	101-115	95-107	86-97
1:8	Average	108	101	91

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GAD1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GAD1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GAD1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human GAD1. You can calculate the concentration of Human GAD1 in the samples by comparing the RLU value of the samples to the standard curve.

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