Human GAL3 (Galectin 3) CLIA Kit (E-CL-H0948)

Name: Human GAL3 (Galectin 3) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0948
Price: 180.0 USD
Availability: InStock

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Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human GAL3 in samples.No significant cross-reactivity or interference between Human GAL3 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	59118.000	61888.000	60503.000	60462
1000	23177.000	27935.000	25556.000	25515
500	12304.000	11558.000	11931.000	11890
250	5020.000	5640.000	5330.000	5289
125	2924.000	2686.000	2805.000	2764
62.5	1379.000	1335.000	1357.000	1316
31.25	698.000	730.000	714.000	673
0	40.000	42.000	41.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	104.640	302.320	798.060	110.520	316.190	728.100
Standard deviation	11.300	25.150	72.460	12.880	30.700	74.410
CV（%）	10.800	8.320	9.080	11.650	9.710	10.220

Recovery

The recovery of Human GAL3 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	95-111	101
EDTA plasma （n=8）	87-100	94
Cell culture media （n=8）	93-108	99

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human GAL3 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	88-100	101-114	100-116
1:2	Average	93	107	106
1:4	Range	101-117	91-106	89-104
1:4	Average	107	98	96
1:8	Range	92-103	93-106	89-103
1:8	Average	97	101	94

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GAL3. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GAL3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GAL3, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human GAL3. You can calculate the concentration of Human GAL3 in the samples by comparing the RLU value of the samples to the standard curve.

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