Human GAL7 (Galectin 7) CLIA Kit (E-CL-H0711)

Name: Human GAL7 (Galectin 7) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0711
Price: 180.0 USD
Availability: InStock

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Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human GAL7 in samples.No significant cross-reactivity or interference between Human GAL7 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	58930.000	60714.000	59822.000	59786
1000	23394.000	28450.000	25922.000	25886
500	12208.000	11952.000	12080.000	12044
250	5612.000	5778.000	5695.000	5659
125	2776.000	2502.000	2639.000	2603
62.5	1461.000	1281.000	1371.000	1335
31.25	655.000	717.000	686.000	650
0	35.000	37.000	36.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	106.370	200.680	875.290	103.330	195.520	902.980
Standard deviation	13.030	23.640	81.660	10.090	22.370	102.670
CV（%）	12.250	11.780	9.330	9.760	11.440	11.370

Recovery

The recovery of Human GAL7 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	89-102	95
EDTA plasma （n=8）	97-113	105
Cell culture media （n=8）	85-98	92

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human GAL7 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	98-114	90-106	99-113
1:2	Average	105	97	106
1:4	Range	87-97	87-100	101-117
1:4	Average	92	93	109
1:8	Range	103-116	98-114	96-111
1:8	Average	110	106	102

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GAL7. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GAL7 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GAL7, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human GAL7. You can calculate the concentration of Human GAL7 in the samples by comparing the RLU value of the samples to the standard curve.

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