Human GDH/GLDH (Glutamate dehydrogenase) CLIA Kit (E-CL-H0869)

Name: Human GDH/GLDH (Glutamate dehydrogenase) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0869
Price: 180.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Human GDH/GLDH (Glutamate dehydrogenase) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	0.09 mIU/mL
Detection Range	0.16-10 mIU/mL
Sample Volume	100 μL
Total Assay Time	4 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human GDH/GLDH in samples.No significant cross-reactivity or interference between Human GDH/GLDH and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

mIU/mL	OD1	OD2	Mean OD	Corrected OD
10	32757.000	35177.000	33967.000	33931
5	14626.000	16354.000	15490.000	15454
2.5	8492.000	7968.000	8230.000	8194
1.25	3736.000	3836.000	3786.000	3750
0.63	1925.000	1669.000	1797.000	1761
0.31	935.000	803.000	869.000	833
0.16	367.000	409.000	388.000	352
0	36.000	36.000	36.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（mIU/mL）	0.470	1.240	3.810	0.490	1.250	3.950
Standard deviation	0.050	0.130	0.360	0.040	0.130	0.410
CV（%）	10.640	10.480	9.450	8.160	10.400	10.380

Recovery

The recovery of Human GDH was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	90-107	97
EDTA plasma （n=8）	89-104	97
Cell culture media （n=8）	88-102	94

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human GDH and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	90-104	92-106	95-113
1:2	Average	97	98	103
1:4	Range	86-99	92-103	89-100
1:4	Average	92	97	94
1:8	Range	91-105	103-118	90-102
1:8	Average	98	109	96

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GDH/GLDH. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GDH/GLDH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GDH/GLDH, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human GDH/GLDH. You can calculate the concentration of Human GDH/GLDH in the samples by comparing the RLU value of the samples to the standard curve.