Human GREM1 (Gremlin 1) CLIA Kit (E-CL-H0888)

Name: Human GREM1 (Gremlin 1) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0888
Price: 180.0 USD
Availability: InStock

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Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human GREM1 in samples.No significant cross-reactivity or interference between Human GREM1 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	55212.000	66334.000	60773.000	60748
1000	23134.000	26586.000	24860.000	24835
500	12457.000	10407.000	11432.000	11407
250	5485.000	5895.000	5690.000	5665
125	2847.000	2777.000	2812.000	2787
62.5	1442.000	1328.000	1385.000	1360
31.25	722.000	732.000	727.000	702
0	24.000	26.000	25.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	95.990	259.380	913.080	89.990	235.010	883.200
Standard deviation	10.100	23.500	82.450	7.330	26.230	86.290
CV（%）	10.520	9.060	9.030	8.150	11.160	9.770

Recovery

The recovery of Human GREM1 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	98-112	104
EDTA plasma （n=8）	89-99	94
Cell culture media （n=8）	100-119	108

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human GREM1 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	87-98	93-109	94-109
1:2	Average	93	101	100
1:4	Range	97-108	90-104	92-109
1:4	Average	102	97	100
1:8	Range	89-103	101-115	97-112
1:8	Average	96	108	104

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GREM1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GREM1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GREM1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human GREM1. You can calculate the concentration of Human GREM1 in the samples by comparing the RLU value of the samples to the standard curve.

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