Human GSTpi (Glutathione S Transferases Pi) CLIA Kit (E-CL-H0879)

Name: Human GSTpi (Glutathione S Transferases Pi) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0879
Price: 180.0 USD
Availability: InStock

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Human GSTpi (Glutathione S Transferases Pi) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	0.28 ng/mL
Detection Range	0.47-30 ng/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human GSTpi in samples.No significant cross-reactivity or interference between Human GSTpi and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
30	33363.000	37867.000	35615.000	35585
15	14010.000	15380.000	14695.000	14665
7.5	6607.000	5645.000	6126.000	6096
3.75	2738.000	3184.000	2961.000	2931
1.88	1553.000	1505.000	1529.000	1499
0.94	1015.000	879.000	947.000	917
0.47	652.000	686.000	669.000	639
0	30.000	30.000	30.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	1.620	3.830	12.770	1.500	4.000	12.010
Standard deviation	0.180	0.440	1.360	0.180	0.400	1.290
CV（%）	11.110	11.490	10.650	12.000	10.000	10.740

Recovery

The recovery of Human GSTpi was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	87-103	95
EDTA plasma （n=8）	100-111	105
Cell culture media （n=8）	100-115	106

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human GSTpi and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	94-110	101-117	87-98
1:2	Average	101	107	93
1:4	Range	85-100	94-106	96-110
1:4	Average	91	101	103
1:8	Range	98-115	85-99	83-95
1:8	Average	105	92	90

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GSTpi. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GSTpi and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GSTpi, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human GSTpi. You can calculate the concentration of Human GSTpi in the samples by comparing the RLU value of the samples to the standard curve.

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