Human HLA-A (Leukocyte Antigen A) CLIA Kit (E-CL-H1053)

Name: Human HLA-A (Leukocyte Antigen A) CLIA Kit
Brand: Elabscience
SKU: E-CL-H1053
Price: 180.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Human HLA-A (Leukocyte Antigen A) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	37.5 pg/mL
Detection Range	62.5-4000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human HLA-A in samples.No significant cross-reactivity or interference between Human HLA-A and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
4000	63793.000	65029.000	64411.000	64370
2000	27260.000	30984.000	29122.000	29081
1000	14733.000	13197.000	13965.000	13924
500	6519.000	6757.000	6638.000	6597
250	3635.000	3197.000	3416.000	3375
125	1741.000	1497.000	1619.000	1578
62.5	784.000	830.000	807.000	766
0	40.000	42.000	41.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	185.010	435.080	1940.990	179.600	444.340	1834.060
Standard deviation	19.930	43.160	131.600	16.060	31.190	137.740
CV（%）	10.770	9.920	6.780	8.940	7.020	7.510

Recovery

The recovery of Human HLA-A was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	90-105	97
EDTA plasma （n=8）	100-116	106
Cell culture media （n=8）	85-100	92

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human HLA-A and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	98-115	86-98	95-110
1:2	Average	105	92	101
1:4	Range	101-116	92-106	93-104
1:4	Average	109	97	99
1:8	Range	99-113	100-117	93-107
1:8	Average	106	108	98

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human HLA-A. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HLA-A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HLA-A, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human HLA-A. You can calculate the concentration of Human HLA-A in the samples by comparing the RLU value of the samples to the standard curve.

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