Human HLA-B27 (Human Leukocyte Antigen B27) CLIA Kit (E-CL-H0147)

Name: Human HLA-B27 (Human Leukocyte Antigen B27) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0147
Price: 180.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Human HLA-B27 (Human Leukocyte Antigen B27) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	37.5 pg/mL
Detection Range	62.5-4000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human HLA-B27 in samples.No significant cross-reactivity or interference between Human HLA-B27 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
4000	58597.000	67677.000	63137.000	63106
2000	27461.000	30035.000	28748.000	28717
1000	14476.000	14292.000	14384.000	14353
500	6203.000	7301.000	6752.000	6721
250	3565.000	3355.000	3460.000	3429
125	1600.000	1566.000	1583.000	1552
62.5	774.000	808.000	791.000	760
0	30.000	32.000	31.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	201.490	406.280	1882.240	201.130	427.960	1976.550
Standard deviation	19.850	39.650	214.950	19.210	36.680	151.600
CV（%）	9.850	9.760	11.420	9.550	8.570	7.670

Recovery

The recovery of Human HLA-B27 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	85-100	92
EDTA plasma （n=8）	90-105	98
Cell culture media （n=8）	87-98	92

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human HLA-B27 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	99-115	96-108	93-106
1:2	Average	105	102	98
1:4	Range	96-107	95-111	93-108
1:4	Average	102	103	100
1:8	Range	93-108	97-111	95-110
1:8	Average	101	104	101

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human HLA-B27. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HLA-B27 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HLA-B27, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human HLA-B27. You can calculate the concentration of Human HLA-B27 in the samples by comparing the RLU value of the samples to the standard curve.

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