Human hs-CRP (high-sensitivity C-reactive protein) CLIA Kit (E-CL-H1451)

Name: Human hs-CRP (high-sensitivity C-reactive protein) CLIA Kit
Brand: Elabscience
SKU: E-CL-H1451
Price: 180.0 USD
Availability: InStock

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Human hs-CRP (high-sensitivity C-reactive protein) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	4.69 pg/mL
Detection Range	7.81-500 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human hs-CRP in samples.No significant cross-reactivity or interference between Human hs-CRP and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
500	50328.000	54856.000	52592.000	52568
250	19194.000	23372.000	21283.000	21259
125	10188.000	8616.000	9402.000	9378
62.5	4619.000	4971.000	4795.000	4771
31.25	2569.000	2341.000	2455.000	2431
15.63	1332.000	1318.000	1325.000	1301
7.81	720.000	820.000	770.000	746
0	23.000	25.000	24.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	27.000	68.980	181.940	29.600	71.220	177.570
Standard deviation	2.840	5.990	18.430	3.550	5.980	19.570
CV（%）	10.520	8.680	10.130	11.990	8.400	11.020

Recovery

The recovery of Human hs-CRP was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	84-96	91
EDTA plasma （n=8）	89-102	94
Cell culture media （n=8）	89-102	94

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human hs-CRP and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	86-100	94-106	91-106
1:2	Average	92	101	98
1:4	Range	96-108	102-119	99-113
1:4	Average	102	109	106
1:8	Range	96-112	99-114	94-111
1:8	Average	104	106	102

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human hs-CRP. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human hs-CRP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human hs-CRP, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human hs-CRP. You can calculate the concentration of Human hs-CRP in the samples by comparing the RLU value of the samples to the standard curve.

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