Human HSP-90 (Heat Shock Protein 90) CLIA Kit (E-CL-H1154)

Name: Human HSP-90 (Heat Shock Protein 90) CLIA Kit
Brand: Elabscience
SKU: E-CL-H1154
Price: 180.0 USD
Availability: InStock

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Human HSP-90 (Heat Shock Protein 90) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	37.5 pg/mL
Detection Range	62.5-4000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human HSP-90 in samples.No significant cross-reactivity or interference between Human HSP-90 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
4000	60021.000	60657.000	60339.000	60300
2000	28496.000	30948.000	29722.000	29683
1000	14109.000	13171.000	13640.000	13601
500	6686.000	6980.000	6833.000	6794
250	3707.000	3161.000	3434.000	3395
125	1640.000	1534.000	1587.000	1548
62.5	787.000	819.000	803.000	764
0	38.000	40.000	39.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	222.050	377.850	1698.930	237.820	375.250	1576.390
Standard deviation	23.320	35.330	156.300	22.140	43.420	166.150
CV（%）	10.500	9.350	9.200	9.310	11.570	10.540

Recovery

The recovery of Human HSP-90 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	91-103	97
EDTA plasma （n=8）	93-107	100
Cell culture media （n=8）	100-117	108

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human HSP-90 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	94-109	86-98	98-113
1:2	Average	100	92	104
1:4	Range	99-114	99-115	99-111
1:4	Average	105	105	106
1:8	Range	100-119	101-117	96-107
1:8	Average	108	109	101

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human HSP-90. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HSP-90 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HSP-90, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human HSP-90. You can calculate the concentration of Human HSP-90 in the samples by comparing the RLU value of the samples to the standard curve.

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