Human IDE (Insulin Degrading Enzyme) CLIA Kit (E-CL-H0349)

Name: Human IDE (Insulin Degrading Enzyme) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0349
Price: 180.0 USD
Availability: InStock

Copy the product info.

Citations ({{ citationsPage.numTotal }}) Manual MSDS

Human IDE (Insulin Degrading Enzyme) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

Bulk request

Add to cart

For research use only. Order now, ship in 3 days

Product Summary

Sensitivity	9.38 pg/mL
Detection Range	15.63-1000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human IDE in samples.No significant cross-reactivity or interference between Human IDE and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
1000	50336.000	59630.000	54983.000	54949
500	23425.000	23677.000	23551.000	23517
250	10885.000	10521.000	10703.000	10669
125	4728.000	5454.000	5091.000	5057
62.5	2590.000	2342.000	2466.000	2432
31.25	1405.000	1209.000	1307.000	1273
15.63	737.000	769.000	753.000	719
0	33.000	35.000	34.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	49.470	111.520	391.000	48.690	108.490	395.330
Standard deviation	6.330	9.320	25.420	4.270	10.660	43.370
CV（%）	12.800	8.360	6.500	8.770	9.830	10.970

Recovery

The recovery of Human IDE was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	86-99	92
EDTA plasma （n=8）	98-113	104
Cell culture media （n=8）	88-101	94

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human IDE and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	86-97	94-108	91-105
1:2	Average	91	99	98
1:4	Range	103-117	87-101	101-116
1:4	Average	109	93	107
1:8	Range	91-103	98-111	87-101
1:8	Average	96	104	92

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IDE. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IDE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IDE, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human IDE. You can calculate the concentration of Human IDE in the samples by comparing the RLU value of the samples to the standard curve.

Background