Human IFNα(Interferon Alpha) CLIA Kit (E-CL-H1455)

Name: Human IFNα(Interferon Alpha) CLIA Kit
Brand: Elabscience
SKU: E-CL-H1455
Price: 180.0 USD
Availability: InStock

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Human IFNα(Interferon Alpha) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	4.69 pg/mL
Detection Range	7.81-500 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human IFNα in samples.No significant cross-reactivity or interference between Human IFNα and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
500	34942.000	33152.000	34047.000	33991
250	17415.000	17247.000	17331.000	17275
125	7903.000	7591.000	7747.000	7691
62.5	3617.000	3609.000	3613.000	3557
31.25	1803.000	1813.000	1808.000	1752
15.63	875.000	869.000	872.000	816
7.81	364.000	358.000	361.000	305
0	65.000	47.000	56.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	24.700	50.470	218.780	21.970	61.620	235.230
Standard deviation	1.320	2.480	11.860	1.310	2.940	11.270
CV（%）	5.340	4.910	5.420	5.960	4.770	4.790

Recovery

The recovery of Human IFNα was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	87-100	94
EDTA plasma （n=8）	100-108	103
Cell culture media （n=8）	98-108	102

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human IFNα and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	94-105	100-113	87-98
1:2	Average	98	105	90
1:4	Range	91-103	91-102	92-106
1:4	Average	96	99	98
1:8	Range	84-100	94-105	98-111
1:8	Average	92	101	105

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IFNα. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IFNα and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IFNα, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human IFNα. You can calculate the concentration of Human IFNα in the samples by comparing the RLU value of the samples to the standard curve.

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