Human IgE (Immunoglobulin E) CLIA Kit (E-CL-H1284)

Name: Human IgE (Immunoglobulin E) CLIA Kit
Brand: Elabscience
SKU: E-CL-H1284
Price: 180.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Human IgE (Immunoglobulin E) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	0.38 ng/mL
Detection Range	0.63-40 ng/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human IgE in samples.No significant cross-reactivity or interference between Human IgE and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
40	36889.000	43351.000	40120.000	40086
20	14452.000	14540.000	14496.000	14462
10	6791.000	6739.000	6765.000	6731
5	3040.000	3506.000	3273.000	3239
2.5	1667.000	1613.000	1640.000	1606
1.25	1028.000	928.000	978.000	944
0.63	583.000	651.000	617.000	583
0	34.000	34.000	34.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	1.990	3.770	16.290	2.100	3.420	17.030
Standard deviation	0.250	0.390	1.300	0.250	0.370	1.140
CV（%）	12.560	10.340	7.980	11.900	10.820	6.690

Recovery

The recovery of Human IgE was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	99-113	106
EDTA plasma （n=8）	95-111	103
Cell culture media （n=8）	90-101	95

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human IgE and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	101-118	104-120	88-102
1:2	Average	109	110	95
1:4	Range	101-117	85-97	89-101
1:4	Average	107	90	95
1:8	Range	88-100	96-111	95-106
1:8	Average	93	102	100

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IgE. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IgE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IgE, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human IgE. You can calculate the concentration of Human IgE in the samples by comparing the RLU value of the samples to the standard curve.

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