Human IGFBP-1 (Insulin Like Growth Factor Binding Protein 1) CLIA Kit (E-CL-H0361)

Name: Human IGFBP-1 (Insulin Like Growth Factor Binding Protein 1) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0361
Price: 180.0 USD
Availability: InStock

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Human IGFBP-1 (Insulin Like Growth Factor Binding Protein 1) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human IGFBP-1 in samples.No significant cross-reactivity or interference between Human IGFBP-1 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	59025.000	62449.000	60737.000	60705
1000	26295.000	26393.000	26344.000	26312
500	12051.000	10915.000	11483.000	11451
250	5621.000	5747.000	5684.000	5652
125	2950.000	2674.000	2812.000	2780
62.5	1419.000	1339.000	1379.000	1347
31.25	664.000	762.000	713.000	681
0	31.000	33.000	32.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	100.380	194.910	799.380	104.450	186.910	801.010
Standard deviation	8.310	21.480	87.130	9.670	15.140	84.350
CV（%）	8.280	11.020	10.900	9.260	8.100	10.530

Recovery

The recovery of Human IGFBP-1 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	90-107	97
EDTA plasma （n=8）	92-106	97
Cell culture media （n=8）	94-109	100

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human IGFBP-1 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	92-102	100-114	90-104
1:2	Average	97	106	96
1:4	Range	86-99	99-114	102-116
1:4	Average	93	106	110
1:8	Range	96-109	86-99	101-118
1:8	Average	102	93	108

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IGFBP-1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IGFBP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IGFBP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human IGFBP-1. You can calculate the concentration of Human IGFBP-1 in the samples by comparing the RLU value of the samples to the standard curve.

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