Human INS (Insulin) CLIA Kit (E-CL-H1033)

Name: Human INS (Insulin) CLIA Kit
Brand: Elabscience
SKU: E-CL-H1033
Price: 180.0 USD
Availability: InStock

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Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	0.38 ng/mL
Detection Range	0.63-40 ng/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human INS in samples.No significant cross-reactivity or interference between Human INS and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
40	36208.000	39584.000	37896.000	37861
20	15357.000	15729.000	15543.000	15508
10	6690.000	6128.000	6409.000	6374
5	3202.000	3204.000	3203.000	3168
2.5	1645.000	1539.000	1592.000	1557
1.25	991.000	987.000	989.000	954
0.63	601.000	689.000	645.000	610
0	35.000	35.000	35.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	1.830	6.140	14.270	1.980	6.690	14.090
Standard deviation	0.190	0.560	0.940	0.230	0.740	0.920
CV（%）	10.380	9.120	6.590	11.620	11.060	6.530

Recovery

The recovery of Human INS was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	90-101	96
EDTA plasma （n=8）	85-99	92
Cell culture media （n=8）	97-112	103

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human INS and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	98-113	94-109	100-115
1:2	Average	106	100	106
1:4	Range	88-102	96-110	87-98
1:4	Average	94	103	92
1:8	Range	98-114	90-104	103-118
1:8	Average	105	96	109

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human INS. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human INS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human INS, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human INS. You can calculate the concentration of Human INS in the samples by comparing the RLU value of the samples to the standard curve.

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