Human KIM-1 (Kidney Injury Molecule 1) CLIA Kit (E-CL-H0170)

Name: Human KIM-1 (Kidney Injury Molecule 1) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0170
Price: 180.0 USD
Availability: InStock

Copy the product info.

Citations ({{ citationsPage.numTotal }}) Manual MSDS

Human KIM-1 (Kidney Injury Molecule 1) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

Bulk request

Add to cart

For research use only. Order now, ship in 3 days

Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human KIM-1 in samples.No significant cross-reactivity or interference between Human KIM-1 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	57553.000	60037.000	58795.000	58774
1000	25221.000	26207.000	25714.000	25693
500	12258.000	11802.000	12030.000	12009
250	5160.000	5752.000	5456.000	5435
125	2980.000	2592.000	2786.000	2765
62.5	1410.000	1330.000	1370.000	1349
31.25	680.000	726.000	703.000	682
0	20.000	22.000	21.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	94.140	183.760	955.580	98.230	189.210	1032.400
Standard deviation	7.670	15.910	66.030	12.760	20.640	85.380
CV（%）	8.150	8.660	6.910	12.990	10.910	8.270

Recovery

The recovery of Human KIM-1 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	90-105	96
EDTA plasma （n=8）	94-110	100
Cell culture media （n=8）	97-111	103

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human KIM-1 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	92-108	96-110	87-103
1:2	Average	99	104	94
1:4	Range	86-100	103-118	85-99
1:4	Average	92	109	92
1:8	Range	91-106	97-114	91-105
1:8	Average	97	105	96

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human KIM-1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human KIM-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human KIM-1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human KIM-1. You can calculate the concentration of Human KIM-1 in the samples by comparing the RLU value of the samples to the standard curve.

Background