Human MMP-9 (Matrix Metalloproteinase 9) CLIA Kit (E-CL-H0936)

Name: Human MMP-9 (Matrix Metalloproteinase 9) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0936
Price: 180.0 USD
Availability: InStock

Copy the product info.

Citations ({{ citationsPage.numTotal }}) Manual MSDS

Human MMP-9 (Matrix Metalloproteinase 9) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

Bulk request

Add to cart

For research use only. Order now, ship in 3 days

Product Summary

Sensitivity	37.5 pg/mL
Detection Range	62.5-4000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human MMP-9 in samples.No significant cross-reactivity or interference between Human MMP-9 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
4000	63928.000	65730.000	64829.000	64795
2000	28355.000	28547.000	28451.000	28417
1000	14782.000	13374.000	14078.000	14044
500	6458.000	7072.000	6765.000	6731
250	3778.000	3276.000	3527.000	3493
125	1826.000	1644.000	1735.000	1701
62.5	728.000	834.000	781.000	747
0	34.000	34.000	34.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	220.890	625.040	1419.980	223.420	687.020	1340.570
Standard deviation	20.760	47.380	127.090	23.590	74.540	142.370
CV（%）	9.400	7.580	8.950	10.560	10.850	10.620

Recovery

The recovery of Human MMP-9 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	98-112	104
EDTA plasma （n=8）	92-109	99
Cell culture media （n=8）	102-116	107

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human MMP-9 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	89-104	101-116	97-114
1:2	Average	95	108	105
1:4	Range	89-106	95-108	96-110
1:4	Average	97	103	102
1:8	Range	86-97	87-100	91-103
1:8	Average	91	92	97

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MMP-9. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MMP-9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MMP-9, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human MMP-9. You can calculate the concentration of Human MMP-9 in the samples by comparing the RLU value of the samples to the standard curve.

Background