Human MRP1 (Multidrug Resistance Associated Protein 1) CLIA Kit (E-CL-H1220)

Name: Human MRP1 (Multidrug Resistance Associated Protein 1) CLIA Kit
Brand: Elabscience
SKU: E-CL-H1220
Price: 180.0 USD
Availability: InStock

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Human MRP1 (Multidrug Resistance Associated Protein 1) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	37.5 pg/mL
Detection Range	62.5-4000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human MRP1 in samples.No significant cross-reactivity or interference between Human MRP1 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
4000	54711.000	63637.000	59174.000	59141
2000	29460.000	31364.000	30412.000	30379
1000	14547.000	13317.000	13932.000	13899
500	6662.000	7866.000	7264.000	7231
250	3761.000	3345.000	3553.000	3520
125	1697.000	1621.000	1659.000	1626
62.5	764.000	864.000	814.000	781
0	33.000	33.000	33.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	200.990	449.130	1771.680	219.190	485.850	1899.140
Standard deviation	20.600	35.980	181.950	18.460	40.080	179.850
CV（%）	10.250	8.010	10.270	8.420	8.250	9.470

Recovery

The recovery of Human MRP1 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	93-107	99
EDTA plasma （n=8）	93-107	100
Cell culture media （n=8）	94-108	101

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human MRP1 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	84-98	101-115	88-99
1:2	Average	90	109	94
1:4	Range	97-113	100-114	89-103
1:4	Average	104	107	95
1:8	Range	98-111	97-109	99-111
1:8	Average	105	102	104

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MRP1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MRP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MRP1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human MRP1. You can calculate the concentration of Human MRP1 in the samples by comparing the RLU value of the samples to the standard curve.

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