Human MUC4 (Mucin 4) CLIA Kit (E-CL-H0519)

Name: Human MUC4 (Mucin 4) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0519
Price: 180.0 USD
Availability: InStock

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Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	37.5 pg/mL
Detection Range	62.5-4000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human MUC4 in samples.No significant cross-reactivity or interference between Human MUC4 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
4000	56786.000	63788.000	60287.000	60251
2000	27837.000	28705.000	28271.000	28235
1000	14806.000	12508.000	13657.000	13621
500	6599.000	7719.000	7159.000	7123
250	3643.000	3221.000	3432.000	3396
125	1738.000	1624.000	1681.000	1645
62.5	761.000	763.000	762.000	726
0	35.000	37.000	36.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	204.660	626.410	1420.500	209.120	675.430	1549.060
Standard deviation	24.290	48.110	105.690	20.790	62.270	128.730
CV（%）	11.870	7.680	7.440	9.940	9.220	8.310

Recovery

The recovery of Human MUC4 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	90-105	98
EDTA plasma （n=8）	85-97	91
Cell culture media （n=8）	90-104	98

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human MUC4 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	85-99	96-110	86-99
1:2	Average	91	102	93
1:4	Range	89-103	93-109	102-116
1:4	Average	95	101	108
1:8	Range	92-106	93-105	94-109
1:8	Average	97	100	101

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MUC4. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MUC4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MUC4, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human MUC4. You can calculate the concentration of Human MUC4 in the samples by comparing the RLU value of the samples to the standard curve.

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