Human MUC5B (Mucin 5 Subtype B) CLIA Kit (E-CL-H1341)

Name: Human MUC5B (Mucin 5 Subtype B) CLIA Kit
Brand: Elabscience
SKU: E-CL-H1341
Price: 180.0 USD
Availability: InStock

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Human MUC5B (Mucin 5 Subtype B) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	75 pg/mL
Detection Range	125-8000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human MUC5B in samples.No significant cross-reactivity or interference between Human MUC5B and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
8000	69044.000	74324.000	71684.000	71644
4000	33129.000	37867.000	35498.000	35458
2000	17883.000	17669.000	17776.000	17736
1000	8743.000	9363.000	9053.000	9013
500	4329.000	4085.000	4207.000	4167
250	2018.000	1798.000	1908.000	1868
125	728.000	770.000	749.000	709
0	39.000	41.000	40.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	373.500	1161.500	3146.620	354.730	1164.490	2991.280
Standard deviation	33.880	100.930	200.750	38.420	117.850	314.080
CV（%）	9.070	8.690	6.380	10.830	10.120	10.500

Recovery

The recovery of Human MUC5B was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	87-101	92
EDTA plasma （n=8）	89-101	94
Cell culture media （n=8）	92-107	98

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human MUC5B and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	103-116	95-112	99-113
1:2	Average	110	102	104
1:4	Range	94-108	89-104	95-110
1:4	Average	102	97	101
1:8	Range	87-98	90-103	85-95
1:8	Average	92	98	90

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MUC5B. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MUC5B and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MUC5B, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human MUC5B. You can calculate the concentration of Human MUC5B in the samples by comparing the RLU value of the samples to the standard curve.

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