Human MYBPC3 (Myosin Binding Protein C, Cardiac) CLIA Kit (E-CL-H0521)

Name: Human MYBPC3 (Myosin Binding Protein C, Cardiac) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0521
Price: 180.0 USD
Availability: InStock

Copy the product info.

Citations ({{ citationsPage.numTotal }}) Manual MSDS

Human MYBPC3 (Myosin Binding Protein C, Cardiac) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

Bulk request

Add to cart

For research use only. Order now, ship in 3 days

Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human MYBPC3 in samples.No significant cross-reactivity or interference between Human MYBPC3 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	53497.000	64267.000	58882.000	58849
1000	23687.000	28185.000	25936.000	25903
500	12649.000	10561.000	11605.000	11572
250	5196.000	5598.000	5397.000	5364
125	2680.000	2662.000	2671.000	2638
62.5	1465.000	1267.000	1366.000	1333
31.25	693.000	717.000	705.000	672
0	33.000	33.000	33.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	104.540	184.960	687.680	99.780	180.800	704.400
Standard deviation	10.200	15.480	72.210	9.470	16.250	60.440
CV（%）	9.760	8.370	10.500	9.490	8.990	8.580

Recovery

The recovery of Human MYBPC3 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	86-97	92
EDTA plasma （n=8）	97-111	103
Cell culture media （n=8）	95-112	102

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human MYBPC3 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	103-115	102-121	85-100
1:2	Average	109	110	92
1:4	Range	98-113	84-98	103-118
1:4	Average	105	91	109
1:8	Range	98-117	94-108	92-105
1:8	Average	106	100	97

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MYBPC3. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MYBPC3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MYBPC3, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human MYBPC3. You can calculate the concentration of Human MYBPC3 in the samples by comparing the RLU value of the samples to the standard curve.

Background