Human MYO (Myoglobin) CLIA Kit (E-CL-H0134)

Name: Human MYO (Myoglobin) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0134
Price: 180.0 USD
Availability: InStock

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Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	0.38 ng/mL
Detection Range	0.63-40 ng/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human MYO in samples.No significant cross-reactivity or interference between Human MYO and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
40	34608.000	38912.000	36760.000	36733
20	14331.000	15469.000	14900.000	14873
10	6745.000	6427.000	6586.000	6559
5	3027.000	3373.000	3200.000	3173
2.5	1675.000	1569.000	1622.000	1595
1.25	1038.000	960.000	999.000	972
0.63	616.000	678.000	647.000	620
0	26.000	28.000	27.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	2.220	5.240	18.710	2.200	5.390	20.370
Standard deviation	0.260	0.610	1.390	0.270	0.540	2.230
CV（%）	11.710	11.640	7.430	12.270	10.020	10.950

Recovery

The recovery of Human MYO was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	89-102	95
EDTA plasma （n=8）	88-103	96
Cell culture media （n=8）	100-113	107

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human MYO and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	97-111	85-97	87-99
1:2	Average	105	91	93
1:4	Range	97-112	97-114	86-98
1:4	Average	105	105	91
1:8	Range	93-107	101-113	93-105
1:8	Average	100	107	98

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MYO. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MYO and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MYO, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human MYO. You can calculate the concentration of Human MYO in the samples by comparing the RLU value of the samples to the standard curve.

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