Human NGAL (Neutrophil Gelatinase Associated Lipocalin) CLIA Kit (E-CL-H0095)

Name: Human NGAL (Neutrophil Gelatinase Associated Lipocalin) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0095
Price: 180.0 USD
Availability: InStock

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Human NGAL (Neutrophil Gelatinase Associated Lipocalin) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human NGAL in samples.No significant cross-reactivity or interference between Human NGAL and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	54671.000	56945.000	55808.000	55769
1000	23524.000	28220.000	25872.000	25833
500	12330.000	11464.000	11897.000	11858
250	5624.000	5862.000	5743.000	5704
125	2951.000	2593.000	2772.000	2733
62.5	1471.000	1383.000	1427.000	1388
31.25	651.000	707.000	679.000	640
0	37.000	41.000	39.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	100.800	190.470	894.540	92.780	207.900	905.470
Standard deviation	10.150	19.120	67.630	11.660	18.860	81.220
CV（%）	10.070	10.040	7.560	12.570	9.070	8.970

Recovery

The recovery of Human NGAL was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	98-109	104
EDTA plasma （n=8）	97-111	103
Cell culture media （n=8）	93-103	98

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human NGAL and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	96-113	99-113	91-105
1:2	Average	103	107	97
1:4	Range	86-100	93-106	102-115
1:4	Average	93	100	109
1:8	Range	99-114	87-103	95-111
1:8	Average	105	94	102

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human NGAL. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NGAL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NGAL, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human NGAL. You can calculate the concentration of Human NGAL in the samples by comparing the RLU value of the samples to the standard curve.

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