Human NT-3 (Neurotrophin-3) CLIA Kit (E-CL-H1171)

Name: Human NT-3 (Neurotrophin-3) CLIA Kit
Brand: Elabscience
SKU: E-CL-H1171
Price: 180.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Human NT-3 (Neurotrophin-3) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	9.38 pg/mL
Detection Range	15.63-1000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human NT-3 in samples.No significant cross-reactivity or interference between Human NT-3 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
1000	54364.000	60484.000	57424.000	57394
500	21823.000	24795.000	23309.000	23279
250	10730.000	10654.000	10692.000	10662
125	4928.000	5358.000	5143.000	5113
62.5	2712.000	2474.000	2593.000	2563
31.25	1430.000	1260.000	1345.000	1315
15.63	691.000	733.000	712.000	682
0	29.000	31.000	30.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	53.730	84.810	399.670	51.470	80.060	421.550
Standard deviation	4.530	9.770	37.370	5.410	9.540	36.840
CV（%）	8.430	11.520	9.350	10.510	11.920	8.740

Recovery

The recovery of Human NT-3 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	96-112	103
EDTA plasma （n=8）	98-116	106
Cell culture media （n=8）	102-118	108

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human NT-3 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	90-102	88-98	90-101
1:2	Average	96	93	96
1:4	Range	88-100	91-104	96-110
1:4	Average	95	97	102
1:8	Range	87-99	93-108	99-112
1:8	Average	92	100	105

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human NT-3. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NT-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NT-3, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human NT-3. You can calculate the concentration of Human NT-3 in the samples by comparing the RLU value of the samples to the standard curve.

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