Human OSMR (Oncostatin M Receptor) CLIA Kit (E-CL-H1347)

Name: Human OSMR (Oncostatin M Receptor) CLIA Kit
Brand: Elabscience
SKU: E-CL-H1347
Price: 180.0 USD
Availability: InStock

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Human OSMR (Oncostatin M Receptor) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human OSMR in samples.No significant cross-reactivity or interference between Human OSMR and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	55534.000	62062.000	58798.000	58775
1000	21894.000	26492.000	24193.000	24170
500	12300.000	11520.000	11910.000	11887
250	4985.000	5859.000	5422.000	5399
125	2991.000	2603.000	2797.000	2774
62.5	1394.000	1378.000	1386.000	1363
31.25	686.000	760.000	723.000	700
0	22.000	24.000	23.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	104.610	243.260	725.400	105.970	257.710	754.980
Standard deviation	10.540	28.170	61.080	11.010	28.810	53.680
CV（%）	10.080	11.580	8.420	10.390	11.180	7.110

Recovery

The recovery of Human OSMR was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	100-116	106
EDTA plasma （n=8）	85-101	92
Cell culture media （n=8）	93-104	98

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human OSMR and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	98-108	103-119	91-108
1:2	Average	103	109	99
1:4	Range	99-116	100-118	92-104
1:4	Average	107	108	97
1:8	Range	100-115	93-107	100-118
1:8	Average	107	99	108

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human OSMR. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human OSMR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human OSMR, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human OSMR. You can calculate the concentration of Human OSMR in the samples by comparing the RLU value of the samples to the standard curve.

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