Human PAF (Platelet Activating Factor) CLIA Kit (E-CL-H1304)

Name: Human PAF (Platelet Activating Factor) CLIA Kit
Brand: Elabscience
SKU: E-CL-H1304
Price: 180.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Human PAF (Platelet Activating Factor) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	37.5 pg/mL
Detection Range	62.5-4000 pg/mL
Sample Volume	100 μL
Total Assay Time	4 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human PAF in samples.No significant cross-reactivity or interference between Human PAF and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
4000	55185.000	67169.000	61177.000	61155
2000	27175.000	32013.000	29594.000	29572
1000	14056.000	13916.000	13986.000	13964
500	6905.000	7231.000	7068.000	7046
250	3674.000	3302.000	3488.000	3466
125	1682.000	1560.000	1621.000	1599
62.5	756.000	760.000	758.000	736
0	21.000	23.000	22.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	221.410	602.650	1403.590	200.310	564.240	1276.200
Standard deviation	21.810	70.270	160.990	26.020	61.730	118.560
CV（%）	9.850	11.660	11.470	12.990	10.940	9.290

Recovery

The recovery of Human PAF was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	92-106	97
EDTA plasma （n=8）	90-106	96
Cell culture media （n=8）	98-112	106

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human PAF and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	91-107	91-108	87-100
1:2	Average	98	99	93
1:4	Range	89-103	89-104	97-114
1:4	Average	95	96	105
1:8	Range	103-115	101-115	88-101
1:8	Average	109	108	94

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PAF. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PAF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PAF, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human PAF. You can calculate the concentration of Human PAF in the samples by comparing the RLU value of the samples to the standard curve.