Human PDGF-BB (Platelet Derived Growth Factor-BB) CLIA Kit (E-CL-H1012)

Name: Human PDGF-BB (Platelet Derived Growth Factor-BB) CLIA Kit
Brand: Elabscience
SKU: E-CL-H1012
Price: 180.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Human PDGF-BB (Platelet Derived Growth Factor-BB) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	9.38 pg/mL
Detection Range	15.63-1000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human PDGF-BB in samples.No significant cross-reactivity or interference between Human PDGF-BB and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
1000	52539.000	63731.000	58135.000	58105
500	22411.000	23767.000	23089.000	23059
250	11286.000	9714.000	10500.000	10470
125	4901.000	5777.000	5339.000	5309
62.5	2589.000	2575.000	2582.000	2552
31.25	1445.000	1233.000	1339.000	1309
15.63	724.000	790.000	757.000	727
0	29.000	31.000	30.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	52.180	155.060	370.180	50.530	159.970	399.150
Standard deviation	4.670	15.600	31.500	5.290	17.240	27.020
CV（%）	8.950	10.060	8.510	10.470	10.780	6.770

Recovery

The recovery of Human PDGF-BB was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	90-105	96
EDTA plasma （n=8）	97-114	105
Cell culture media （n=8）	87-101	93

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human PDGF-BB and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	97-108	89-100	97-111
1:2	Average	102	94	102
1:4	Range	87-99	101-116	94-108
1:4	Average	93	107	100
1:8	Range	100-114	95-106	93-107
1:8	Average	106	101	98

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PDGF-BB. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PDGF-BB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PDGF-BB, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human PDGF-BB. You can calculate the concentration of Human PDGF-BB in the samples by comparing the RLU value of the samples to the standard curve.

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