Human PGF2α(Prostaglandin F2α) ELISA Kit (E-EL-H1841)

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human PGF2α. During the reaction, Human PGF2α in the sample or standard competes with a fixed amount of Human PGF2α on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human PGF2α. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human PGF2α in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Prostaglandin F2α (PGF2α) is a bioactive substance known primarily for its regulatory effects on a variety of physiological processes. PGF2α has a variety of functions and roles in the human body. Promote muscle cell survival and growth: PGF2α can reduce muscle cell death, thereby promoting muscle cell survival and growth. As an endocrine substance in the menstrual cycle: PGF2α is endocrine by the human uterus and is present in the blood and uterine tissue of the menstrual cycle and is part of the menstrual fluid. As a glaucoma treatment drug: PGF2α has been found to be a novel local anti-glaucoma drug. Its mechanism of action is through the action of matrix metalloproteinases on the ciliary muscle and scler-uveal channels, resulting in relaxation of the ciliary muscle and widening of the muscle space. At the same time, the increase of matrix metalloproteinase activity also reduced the resistance of aqueous humor outflow, resulting in the outflow of aqueous humor through the sclero-uveal channel. Role in fetal development: PGF2α plays an important role in fetal development, particularly in promoting uterine contractions, which PGF2α consistently stimulates in comparison to PGE2. Role in inflammatory response: PGF2α also plays a role in inflammatory response, especially in cardiovascular homeostasis and thermoregulation. Role in traumatic liver disease: PGF2α has also shown its biological activity in traumatic liver disease, particularly in evaluating oxidative stress and lipid peroxidation damage. In summary, PGF2α not only plays an important role in muscle cell survival and growth, endocrine substances in the menstrual cycle, glaucoma treatment, fetal development, and inflammatory response in the human body.