Human PTHrP (Parathyroid Hormone Related Protein) CLIA Kit (E-CL-H0953)

Name: Human PTHrP (Parathyroid Hormone Related Protein) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0953
Price: 180.0 USD
Availability: InStock

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Human PTHrP (Parathyroid Hormone Related Protein) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	7.5 pg/mL
Detection Range	12.5-800 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human PTHrP in samples.No significant cross-reactivity or interference between Human PTHrP and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
800	51729.000	57447.000	54588.000	54558
400	20224.000	23886.000	22055.000	22025
200	11015.000	9237.000	10126.000	10096
100	4781.000	5003.000	4892.000	4862
50	2246.000	2212.000	2229.000	2199
25	1164.000	1064.000	1114.000	1084
12.5	527.000	581.000	554.000	524
0	29.000	31.000	30.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	40.220	117.730	339.300	43.610	114.030	351.740
Standard deviation	5.080	9.500	32.610	4.230	8.110	26.060
CV（%）	12.630	8.070	9.610	9.700	7.110	7.410

Recovery

The recovery of Human PTHrP was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	90-100	95
EDTA plasma （n=8）	86-101	93
Cell culture media （n=8）	96-109	101

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human PTHrP and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	101-115	95-107	104-119
1:2	Average	109	100	110
1:4	Range	103-118	103-116	99-117
1:4	Average	109	109	107
1:8	Range	96-111	89-102	99-110
1:8	Average	101	95	104

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PTHrP. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PTHrP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PTHrP, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human PTHrP. You can calculate the concentration of Human PTHrP in the samples by comparing the RLU value of the samples to the standard curve.

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