Human PYGL (Glycogen Phosphorylase, Liver) CLIA Kit (E-CL-H0857)

Name: Human PYGL (Glycogen Phosphorylase, Liver) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0857
Price: 180.0 USD
Availability: InStock

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Human PYGL (Glycogen Phosphorylase, Liver) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	4 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human PYGL in samples.No significant cross-reactivity or interference between Human PYGL and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	57831.000	64275.000	61053.000	61026
1000	24364.000	25396.000	24880.000	24853
500	12012.000	11780.000	11896.000	11869
250	5054.000	5686.000	5370.000	5343
125	2864.000	2630.000	2747.000	2720
62.5	1437.000	1403.000	1420.000	1393
31.25	697.000	741.000	719.000	692
0	26.000	28.000	27.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	102.700	256.040	730.140	107.040	261.880	668.250
Standard deviation	9.240	20.640	63.810	12.350	29.930	66.160
CV（%）	9.000	8.060	8.740	11.540	11.430	9.900

Recovery

The recovery of Human PYGL was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	90-103	97
EDTA plasma （n=8）	93-108	99
Cell culture media （n=8）	95-110	102

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human PYGL and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	94-107	102-119	94-105
1:2	Average	100	109	99
1:4	Range	102-119	102-118	87-103
1:4	Average	108	110	94
1:8	Range	98-110	88-101	85-101
1:8	Average	104	93	92

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PYGL. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PYGL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PYGL, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human PYGL. You can calculate the concentration of Human PYGL in the samples by comparing the RLU value of the samples to the standard curve.