Human RNASE3/ECP (Ribonuclease A3/Eosinophil Cationic Protein) CLIA Kit (E-CL-H0898)

Name: Human RNASE3/ECP (Ribonuclease A3/Eosinophil Cationic Protein) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0898
Price: 180.0 USD
Availability: InStock

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Human RNASE3/ECP (Ribonuclease A3/Eosinophil Cationic Protein) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	7.5 pg/mL
Detection Range	12.5-800 pg/mL
Sample Volume	100 μL
Total Assay Time	4 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human RNASE3/ECP in samples.No significant cross-reactivity or interference between Human RNASE3/ECP and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
800	51941.000	58083.000	55012.000	54982
400	21880.000	24642.000	23261.000	23231
200	11487.000	9823.000	10655.000	10625
100	4691.000	4813.000	4752.000	4722
50	2313.000	2231.000	2272.000	2242
25	1226.000	1056.000	1141.000	1111
12.5	517.000	523.000	520.000	490
0	29.000	31.000	30.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	42.420	78.120	275.700	41.660	84.510	257.790
Standard deviation	5.430	5.910	19.440	4.400	6.480	17.920
CV（%）	12.800	7.570	7.050	10.560	7.670	6.950

Recovery

The recovery of Human RNASE3 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	86-99	93
EDTA plasma （n=8）	92-104	98
Cell culture media （n=8）	100-114	108

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human RNASE3 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	89-99	89-103	95-109
1:2	Average	94	96	101
1:4	Range	95-111	101-120	88-98
1:4	Average	102	109	93
1:8	Range	94-110	102-118	95-111
1:8	Average	100	109	101

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human RNASE3/ECP. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human RNASE3/ECP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human RNASE3/ECP, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human RNASE3/ECP. You can calculate the concentration of Human RNASE3/ECP in the samples by comparing the RLU value of the samples to the standard curve.