Human S100A13 (S100 Calcium Binding Protein A13) CLIA Kit (E-CL-H0845)

Name: Human S100A13 (S100 Calcium Binding Protein A13) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0845
Price: 180.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Human S100A13 (S100 Calcium Binding Protein A13) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	37.5 pg/mL
Detection Range	62.5-4000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human S100A13 in samples.No significant cross-reactivity or interference between Human S100A13 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
4000	57542.000	69494.000	63518.000	63479
2000	25621.000	31023.000	28322.000	28283
1000	14818.000	12648.000	13733.000	13694
500	6534.000	6968.000	6751.000	6712
250	3385.000	3215.000	3300.000	3261
125	1728.000	1540.000	1634.000	1595
62.5	709.000	811.000	760.000	721
0	39.000	39.000	39.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	214.640	482.700	1505.110	212.700	479.760	1473.530
Standard deviation	25.330	52.420	143.290	20.630	52.820	92.390
CV（%）	11.800	10.860	9.520	9.700	11.010	6.270

Recovery

The recovery of Human S100A13 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	97-114	105
EDTA plasma （n=8）	96-108	102
Cell culture media （n=8）	91-105	96

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human S100A13 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	96-111	95-107	95-113
1:2	Average	104	101	103
1:4	Range	86-101	88-102	92-106
1:4	Average	92	93	99
1:8	Range	89-103	90-107	99-112
1:8	Average	95	97	106

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human S100A13. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human S100A13 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human S100A13, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human S100A13. You can calculate the concentration of Human S100A13 in the samples by comparing the RLU value of the samples to the standard curve.

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