Human SIRT1 (Sirtuin 1) CLIA Kit (E-CL-H0992)
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For research use only.
Product Summary
| Sensitivity | 37.5 pg/mL |
| Detection Range | 62.5-4000 pg/mL |
| Sample Volume | 100 μL |
| Total Assay Time | 3 h 30 min |
| Reactivity | Human |
| Specificity | This kit recognizes Human SIRT1 in samples.No significant cross-reactivity or interference between Human SIRT1 and analogues was observed |
| Recovery | 80%-120% |
| Sample Type | Tissue homogenates, cell lysates and other biological fluids |
| Detection Method | Chemiluminescence |
| Assay Type | Sandwich-CLIA |
| Size | 96T / 48T / 24T / 96T*5 / 96T*10 |
| Storage | 2-8℃/-20℃ |
| Expiration Date | 6 months |
Test Principle
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SIRT1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SIRT1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SIRT1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human SIRT1. You can calculate the concentration of Human SIRT1 in the samples by comparing the RLU value of the samples to the standard curve.
Background
| Gene ID | 23411 |
| Uniport ID | Q96EB6 |
| Research Area | Cancer , Cell Biology , Epigenetics And Nuclear Signaling , Metabolism , Microbiology , Tags And Cell Markers |
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