Human SLAMF2 (Signaling Lymphocytic Activation Molecule Family, Member 2) ELISA Kit (E-EL-H6315)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SLAMF2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SLAMF2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SLAMF2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 4 nm. The OD value is proportional to the concentration of Human SLAMF2. You can calculate the concentration of Human SLAMF2 in the samples by comparing the OD of the samples to the standard curve.

Glycosylphosphatidylinositol (GPI)-anchored cell surface glycoprotein interacts via its N-terminal immunoglobulin domain with cell surface receptors (e.g., CD244/2B4, CD2) to regulate immune cell function and activation. It participates in T-cell signaling transduction by associating with CD2, efficiently recruiting Src family protein kinase LCK and LAT to the TCR/CD3 complex, thereby promoting LCK phosphorylation and activation. Additionally, it induces phosphorylation of the cytoplasmic immunoreceptor tyrosine switch motifs (ITSMs) of CD244, initiating signaling events that drive immunological synapse formation and directed release of cytolytic granules (containing perforin and granzymes) by T-lymphocytes and NK-cells.